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FcR Binding Assay Services
Measuring an antibody’s affinity to the Fcγ receptors has become an integral part of biotherapeutics development. Syd Labs has established a standard human Fcγ receptor panel binding assay in a high throughput format using the BLI platform. This in vitro binding assay can also be used for quality control purposed in parallel with functional cell-based ADCC. Additionally, Syd Labs offers clients an FcRn binding assay to study pH-dependent interaction of antibody with the neonatal Fc-receptor (FcRn) in high throughput and easy to use format. The outcome of the study will enhance overall understanding of the antibody and demonstrate comparability in the development of biosimilars.
FcRγ binding assay and FcRn binding assay services
The panel is performed using Octet Red96 (ForteBio):
- his-tagged receptors are immobilized onto anti-His biosensors;
- the loaded biosensors are dipped into a dilution series of the antibody;
- the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.
- 100 ug each of purified antibody.
Recombinant Human Fc gamma RIIA Protein
Recombinant Human Fc gamma RIIB/C Protein
Recombinant Human Fc gamma RIIIA Protein (F158)
Recombinant Human Fc gamma RIIIA Protein (V158)
Recombinant Human Fc gamma RIIIB Protein