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EpiArt DNA Methylation Bisulfite Kit
DNA methylation is closely related to gene expression and gene function, and plays a pivotal role in physiological and pathological processes such as genetic imprinting, embryonic development, chromosomal gene silencing and cell cycle regulation.
DNA methylation is closely related to gene expression and gene function, and plays a pivotal role in physiological and pathological processes such as genetic imprinting, embryonic development, chromosomal gene silencing and cell cycle regulation. Efficient and accurate detection of DNA methylation has become increasingly important for biology, genetics, pathology, pharmacology, and medical diagnosis. Bisulfite conversion is a common technique used to study DNA methylation that involves converting unmethylated cytosine bases to uracil bases by treating the DNA with bisulfite, while leaving 5-methylcytosine (5-mC) intact. After conversion, DNA methylation can be determined by PCR amplification or DNA sequencing.
This kit combines DNA denaturation (by thermal denaturation instead of chemical denaturation) and bisulfite conversion into one step, and the conversion reaction time is only 140 min; Using the on-membrane desulphonation technique to expand the amount of DNA input to 100 pg - 2 μg. The recovery efficiency is ≥ 80% and the unmethylated cytosine conversion efficiency is ≥ 99%. The converted product is suitable for downstream applications such as PCR amplification and NGS sequencing.