Mammalian Cell Culture Services

Mammalian Cell Culture Services

CRISPR Genome Editing Services

DNA vectors used in this cell line modification service will NOT be transferred to clients under this arrangement.

CatalogBS019
Grouped product items
Product Name Quantity
Knock-out Cell Line Generation Using CRISPR Genome Editing
$0.00
Knock-in Cell Line Generation Using CRISPR Genome Editing
$0.00

Knock-out and knock-in cell lines are generated by experienced scientists working on lentivirus, adeno-associated virus (AAV), RNAi, and cell line development services for decades. In combination with the powerful CRISPR/Cas9 tool, our proprietary viral system is highly efficient and precise in knock-out and knock-in cell line development with any gene and any host cell, including mammalian primary cell, without introducing foreign DNA.

Generation of knock-out and knock-in cell lines using CRISPR genome editing:

CRISPR/Cas9 DNA constructs are introduced into host cells by viral transduction or non-viral transfection. Knock-out and knock-in cell lines are screened for permanent and precise modification of cellular genome. Our fast and predictive screening techniques may significantly increase speed of knock-out and knock-in cell line development while improving quality and uniformity. The process includes:

- Molecular construction of gRNA, Cas9, and donor vectors based on the target gene/site/locus and cell line specified by clients;
- Virus packaging and production;
- Viral transduction or non-viral transfection of DNA constructs into host cells;
- Selection of cell pool or screening for stable single clones;
- Validation of cell pool or single clones by sequencing and PCR or specific methods such as FACS; (Note: The standard method for validation is sequencing and PCR. Additional fee will be charged for Western blot or cell-based assay of your interest.)
- Batch culture and cell banking.

Service of knock-out cell line generation using CRISPR genome editing includes:

a. Single cell cloning;
b. Detailed clone validation by sequencing;
c. Backup vial storage for 6 months.

Deliverable:
a. The selected clone;
b. Knock-out cell line development study report including gRNA design and sequencing validation results.

Fast turnaround: 16-19 weeks.

Service of knock-in cell line generation using CRISPR genome editing includes:

a. Single cell cloning;
b. Detailed clone validation by sequencing and PCR;
c. Backup vial storage for 6 months.

Deliverable:
a. The selected clone;
b. Knock-in cell line development study report including gRNA design and PCR and sequencing validation results.

Fast turnaround: 20-24 weeks.

Syd Labs also provides the following viral-based cell line generation services:

Lentiviral Based Stable Cell Line Generation Services: development of stable cell lines using lentivirus.
Chimeric Antigen Receptor T-Cell Engineering: development of stable cell lines for precise genomic modification.

Syd Labs also provides the following stable cell line generation services:

Standard Stable Cell Line Development Services: non-viral generation of adhension or suspension stable cell lines with one or two gene insertion.
Stable Cell Line Development Services for Protein Production: development of stable cell lines for recombinant protein expression.
Stable Cell Line Development Services for Antibody Production: development of stable cell lines for recombinant antibody expression.
Stable Cell Line Development Services for Biologics Production: generation of stable cell lines for manufacturing recombinant protein or antibody drugs.

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