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Camelid Monoclonal Antibody Production Services
The lowest prices of custom camel, llama or alpaca VHH antibody production services are guaranteed. If you find any US company providing the same quality services with lower prices, we will return the difference to you.
Procedure of camel, llama and alpaca single chain (VHH) antibody development:
Phase I: Immunization and boosts of 1 camel, Ilama or alpaca, and ELISA screening of camel, Ilama or alpaca polyclonal sera.
Phase II: RNA isolation, cDNA synthesis, PCR amplification, and construction of a phage display library of VHH, a single variable domain (VH) of a heavy chain antibody.
Phase III: Screening antigen-specific VHH by phage display. 4-6 potential clones will be identified and evaluated.
Phase IV: Molecular construction and production of 4-6 ELISA-positive His-tagged VHH protein in E.coli.
You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 5 mg of soluble protein (concentration > 0.5 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.
* DNA sequences of 4-6 antigen-specific VHH;
* Purified His-tagged VHH;
* Remaining antigens;
* ELISA results.
Clients have the choice for licensing antibodies to save cost and even make money.
The prices will be adjusted for the following requirements:
a. Peptide (12-15 amino acids, >85%) design and synthesis +$250 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Synthesis of other antigens, +$variable;
c. Conjugation of antigens to protein carriers +$250;
d. Protein: DNA subcloning +$350, bacterial expression and purification +$2,500;
e. Additional boost immunization for higher ELISA titer of the antisera +$300/injection.
Please see our other monoclonal antibody development services: