Blood Direct PCR Kit is designed to perform PCR directly from whole blood without DNA extraction or sample preparation. This kit can be used to perform PCR amplification from a wide range of templates including fresh blood, 4℃ stored blood, frozen blood, and dried blood stored on Whatman 903 ® or FTA ® commercial card. It is compatible with all conventional anticoagulants (EDTA, citrate, heparin, etc.).
This kit contains Phanta Blood Super-Fidelity DNA Polymerase, which is modified from Phanta Super-Fidelity DNA Polymerase. This enzyme displays superior resistance to the PCR inhibitors in the whole blood sample, and can amplify whole blood with concentration up to 40%. This kit can efficiently amplify genomic fragments of up to 10 kb from whole blood samples with the optimized 2 × Phanta Blood Buffer V2.
Phanta Blood Super-Fidelity DNA Polymerase produces blunt-ended products, which are applicable to ClonExpress cloning Kit (C112/C113). This kit supplies positive control primer mix which is compatible with mammalian and other vertebrate animals and can be used as positive control in experiments.