Monoclonal Antibody Immunoconjugation Service

Monoclonal Antibody Immunoconjugation Service

FcR Binding Assay Services

FcR Binding Assay Services

Antigen-antibody Affinity Measurement Service

CatalogBS073
Grouped product items
Product Name Quantity
Antigen-antibody Affinity Measurement Service by Biacore (SPR) (Catalog No.: BS073A)
Antigen-antibody Affinity Measurement Service by Octet (BLI) (Catalog No.: BS073B)

The non-covalent and reversible antigen-antibody interaction is a combination of hydrogen bonds, hydrophobic interactions, electrostatic and van der Waals forces between an epitope in an antigen and an antibody’s antigen binding site. Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD.

General procedure of antigen-antibody affinity measurement:

A. Antibody is captured on the biacore chip or BLI sensor using an appropriate method.
B. Association and dissociation is measured on the Biacore T100 or the ForteBio Octet QKe using various concentrations of the antigen and the kD is calculated using an appropriate curve-fitting model.

Sample requirements:

A. Antibodies;
B. Antigens.

Deliverable:

A. Study report of antigen-antibody affinity measurement.

Syd Labs provides the antigen production service:
Peptide synthesis service
Recombinant protein production services.

Syd Labs also provides the antibody production service:
Recombinant Antibody (IgG) Production Services
Recombinant Antibody (scFv) Production Services

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