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Albumin Fusion Protein Production Services
No DNAs using our recombinant albumin fusion protein expression vectors will be delivered.
Prices of BS074C to BS074H do not include DNA cloning and scale-up.
Vector DNA construction includes gene synthesis if template DNA is not provided by client. Additional fees apply for gene synthesis over 600 bp.
Syd Labs has extensive experience with making DNA constructs for albumin fusion protein production, expressing and purifying a number of albumin fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized expression and purification process, Syd Labs can efficiently complete a project from DNA construction and transient production of albumin fusion proteins in CHO and HEK 293 cells, to albumin fusion protein purification.
Proteins destined for in vivo use can be fused to albumin to improve plasma half-life and bioavailability due to decreased renal clearance and recycling via the neonatal Fc receptor (FcRn). Different from Fc fusion proteins, albumin fusion proteins are monomeric and do not interact with Fc receptors.
1. DNA construction of albumin-fused proteins:
The gene of interest is synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors. If gene synthesis is requested, free sequence optimization is performed to meet the client's goals such as recombinant albumin-fusion protein expression at high and reliable levels.
2. Expression and purification of albumin-fusion proteins:
* Transiently express the recombinant albumin-fusion protein in pilot small-scale (100 ml) of CHO or HEK 293 cells, followed by custom albumin affinity purification, to assess expression levels and protein yield;
* If requested, scale up transient expression to 0.25 - 20 liters to produce starting material at the milligram to gram scale;
* Purification is performed using a custom albumin affinity purification process. Please request if multiple purification methods are required.
If you provide us the albumin fusion protein expression vector with your gene already cloned into, we perform small-scale pilot expression and protein purification by transient transfection into CHO or 293 cells, allowing us to evaluate expression level and yield of the albumin fusion protein of your interest.
Fast turnaround (BS074A):
* 1-2 weeks for codon optimization and synthesizing the gene of your interest.
* 1-2 weeks for molecular construction of recombinant albumin fusion proteins using E.coli.
We also provide the following protein production (DNA construction, protein expression and purification) services:
Fc Fusion Protein Production Service
His Tag or FLAG Tag Fusion Protein Production Service