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Adenovirus Production Services for Toxic Genes
No DNA using our adenoviral vectors is delivered.
Additional fee is charged if no template of DNA inserts is provided by clients.
Our adenovirus production services for toxic genes include: 1) construction of an adenovirus vector for expression of a cytotoxic gene; 2) generation of adenoviruses carrying cytotoxic genes; 3) amplification and purification of adenoviruses carrying cytotoxic genes.
Some gene products might be cytotoxic to 293 cells, resulting in low yield or failure of adenovirus production. We have a special SpeAd virus system to solve this problem.
With a strong safety record, adenoviruses and recombinant adenoviral vectors have been widely used for gene function studies and human gene therapy. Recombinant adenoviruses that can not replicate are attractive vectors for gene delivery for the following reasons:
- they infect many types of mammalian cells with high efficiency;
- they do not disrupt the genome of the host cell but remain in the nucleus as episomal DNA;
- they provide transient, high level gene expression;
- they are suitable for both in vitro and in vivo applications.
Our adenovirus production team has constructed, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.
BS083A. Molecular construction of an adenovirus vector and generation of an Adenovirus to express a cytotoxic gene.
Your sequence is inserted into the adenoviral genome and transduced into mammalian cells.
A. Crude cell lysate containing the viral construct (approximately 10^9 viral particles/ml), suitable for testing to ensure that the virus performs as expected;
B. Study report.
BS081B. Generation of an adenovirus carrying a cytotoxic gene.
The adenovirus is generated in HEK 293 cells using the adenovirus construct provided by a client.
A. Crude cell lysate containing the viral construct (approximately 10^6 viral particles/ml), suitable for testing to ensure that the virus performs as expected;
B. Study report.
BS081C. Amplification and purification of adenovirus carrying a cytotoxic gene.
Adenovirus vectors supplied by clients or newly constructed by Syd Labs are amplified to 1 or 3 x 10^12 viral particles. The adenoviruses are purified by centrifugation using two sequential cesium chloride gradients. The final products (1 or 3 ml, 1x10^12 viral particles/ml) are dialyzed, titered spectrophotometrically, and tested for sterility.
A. Adenoviral particles;
B. Study report.
Syd Labs also provides the following virus production (virus packaging) services:
Retrovirus Production Services
Adeno-associated Virus (AAV) Production Services
Lentivirus Production Services
Adenovirus Production Services
Adenovirus Production Services for Fiber-modified Ad5-RGD and Ad5/35