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2x Universal SYBR Green qPCR Master Mixes
Conditions of optimal VEGF / VEGF-A / VPF Protein performance should be determined experimentally by the investigator.
Human VEGF-A, also known as vascular endothelial growth factor (VEGF) and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. There are 8 differently spliced isoforms of VEGF, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF-A stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
BP000027-GD1: Recombinant Human VEGF165 (aa 27-191) Protein
Source: HEK 293 cell-derived. Ala27-Arg191, Accession # NP_001165097
N-terminal sequence analysis: Met-Asn.
Structure/Form: Disulfidelinked homodimer.
Predicted molecular mass: 19.2 kDa (monomer).
SDS-PAGE: 17-20 kDa, reducing conditions.
The VEGF activity is measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-5 ng/ml.
Endotoxin: < 1.0 EU per ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions.
Formulation: Lyophilized from a 0.2 um filtered solution with BSA as a carrier protein.
Reconstitution: Reconstitute at 50 µl ddH2O to the concentration of 1.0 mg/ml. To further dilute the VEGF concentration under 10 µg/ml, 1% BSA is recommended..
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
VEGF-A polyclonal antibody
VEGF-B polyclonal antibody
VEGF-D polyclonal antibody
VEGFR2 monoclonal antibody
VEGFR2 (Phospho-Tyr951) polyclonal antibody
VEGFR2 (Phospho-Tyr1214) polyclonal antibody
VEGFR2 (Phospho-Tyr1175) polyclonal antibody
VEGFR2 (Phospho-Tyr1059) polyclonal antibody
VEGFR2 (Phospho-Tyr1054) polyclonal antibody
VEGFR2 polyclonal antibody
VEGFR1 polyclonal antibody
VEGFR1 (Phospho-Tyr1333) polyclonal antibody
VEGFR1 (Phospho-Tyr1048) polyclonal antibody