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Home > Life Sciences > Technique Center > Immunoassay-Western Blotting > Immunofluorescence for Epitope Tagged Proteins

Immunofluorescence for Epitope Tagged Proteins

Wuhan Lobogene Technology, Ltd Updated: October 25, 2008 ID: 000292T0061

Using Ash1-myc as an example, the protocol gives details for the procedure:

Fixing cells;

Spheroplasting;

Prepare slides;

Stick and flatten cells;

Special treatments, Methanol/acetone treatment;

Blocking;

First Antibody Incubation;

Second Antibody Incubation;

Finishing up.

Recipes for solutions are also provided.

Authors: Herskowitz Lab

Acknowledgements:

Notes:

Reference:

Source: Herskowitz Lab

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