Western Blot Protocol from Dr. Frank
The page gives details for the following procedures:
Preparation of Human Cell Extracts;
Preparation of Bacterially Expressed Proteins;
SDS-PAGE Separating Gels (Hoeffer baby gels);
Prepare 10% SDS Stacking (or upper) Gel;
Preparation of Sample;
Electrophoretic Separation of Proteins;
Electrophoretic Transfer for Dry Blots (Millipore);
Electrophoretic Transfer For Wet Blots;
Detection of Antigen on Membranes;
Photograph the membranes with Polaroid Type 57 film;
RECIPES.